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Cost and Turnaround

Price includes in-gel digestion of gel band or spot, nanospray LC/MS/MS analysis, protein identification from a search against the NCBI nonredundant database, and a report emailed to you as a Adobe Acrobat (pdf) file. Prices do not include running (separating) your sample on a gel or excision of gel band or spots (please contact us if you need this done).

In-gel digestions, nanospray LC/MS/MS, and protein identification:

Number of samples Cost
1-4 $250/spot
5-10 was $200
now $150/spot
11-20 was $187.50
now $150/spot
21-40 was $175
now $150/spot
>41 $150/spot

Nanospray LC/MS/MS and protein identification only
(you have already digested your samples):

Number of samples Cost
1-10 $150/sample
11-20 $125/sample
21-50 $100/sample
>50 $85/sample

Note that the typical 1 week turnaround on protein identifications may not always be possible for 20 or more samples.

Phosphorylation, ubiquitination, and other posttranslational modification analysis:

$1000 per sample, per protein, per posttranslational modification. Includes:
  • Optimized in-gel or in-solution digestion with up to two enzymes
  • A report detailing purity of sample (to check if your protein of interest is the major protein in the sample)
  • A report from the initial screen for the posttranslational modification
  • Manual confirmation of up to 4 sites of posttranslational modification on the protein of interest
  • A final report with publication-quality results (includes up to two annotated MS/MS spectra from the observed phosphorylation sites, this is sometime requested by paper reviewers).
If you know the sequence of your protein, please send the sequence before sending any samples so we can evaluate the sequence and choose the optimum enzyme combination. Protein sequences can be sent to us by email.

Shipping address:
Tufts Proteomics
Dept. of Physiology / ST-808
136 Harrison Ave.
Boston, MA 02111

Phone: (617) 636-2422
Fax: (617) 636-6737

All samples must be shipped with completed sample submission forms (see link above to download form).

Print
Sample Submission Forms
Use this sample submission form for nanospray LC/MS/MS based analysis for these type of projects:
  • Protein identification from gel bands, spots, or solution
  • Finding sites of posttranslational modification (phosphorylation, ubiquitination, etc.)
  • Protein characterization projects (confirm n- or c-term, confirm sequence variants, etc.)
  • MudPIT multidimensional LC analysis for complex samples (such as lysates)
Protein ID / PTM / MudPIT
PDF
Use this sample submission for all MALDI-TOF projects including:
  • Molecular weight determination for peptides and proteins
  • Examination of degradation products (by molecular weight)
MALDI-TOF
PDF

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Last Modified: Friday, 22-Aug-2008 14:28:12 EDT
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