MudPIT (Multidimensional Protein Identification Technology) or multi-dimensional LC/MS/MS
MudPIT or multi-dimensional LC/MS/MS analysis is a powerful technique for identifying proteins in a complex mixture (such as a cell lysate, purified organelle fraction, or a mix of 10 proteins or more) that would overwhelm the normal reverse phase LC/MS/MS analysis. MudPIT can also be an alternative to 2D gel electrophoresis for separating complex mixtures of proteins. The MudPIT technique utilizes a single, custom-packed LC column with strong cation exchange (SCX) material packed back-to-back with the standard reversed phase (RP) material. Please call or email to discuss if your sample might benefit from this more detailed form of analysis and the costs of the MudPIT analysis. Please be aware that MudPIT is an involved analysis and requires significant instrument and data analysis time; please allow 3 weeks to complete the full analysis. Normally you don’t need MudPIT separation for IP samples; they usually are not complex enough to need the multistep separation of MudPIT. We usually analyze IP’s by normal LC/MS/MS. Note that MudPIT requires at least 25-100 ug (micrograms) of total protein, depending on sample complexity. MudPIT analysis is not possible on silver stain level samples.
