MudPIT (Multidimensional Protein Identification Technology) or multi-dimensional LC/MS/MS
MudPIT or multi-dimensional LC/MS/MS analysis is a powerful technique for identifying proteins in a complex mixture (such as a cell lysate, purified organelle fraction, or a mix of 10 proteins or more) that would overwhelm the normal reverse phase LC/MS/MS analysis. MudPIT can also be an alternative to 2D gel electrophoresis for separating complex mixtures of proteins. The MudPIT technique utilizes a single, custom-packed LC column with strong cation exchange (SCX) material packed back-to-back with the standard reversed phase (RP) material. Please call or email to discuss if your sample might benefit from this more detailed form of analysis and the costs of the MudPIT analysis.
