Service: Protein identification
We offer high sensitivity LC/MS/MS based protein identification and characterization from gel spots or bands using the latest technologies in mass spectrometry. Our protein characterization services include finding sites of phosphorylation, ubiquitination, oxidation, and other post-translational modifications. Our specialty in protein characterization is the challenging task of finding sites of phosphorylation an ubiquitination. We use the Thermo LTQ ion trap mass spectrometer with nanospray to analyze low amounts (picomole to femtomole) of sample by LC/MS/MS. For complex samples such as lysates or similarly complex protein mixtures, we offer a multidimensional LC (MudPIT) analysis to allow us to identify proteins at lower levels than could be identify by standard LC/MS/MS methods. By comparison and correlation of tandem (MS/MS) mass spectra of peptides with predicted MS/MS spectra generated from protein and nucleotide sequence databases, we can identify proteins in a sample, confirm a sequence, and find sites of post-translational modifications.
Protein Identification Fees
$250 per sample (gel band or spot) for the in-gel digestion procedure and protein identification by LC/MS/MS
Note: Cost above is reduced 20% for internal users. Above cost includes in-gel digestion using a single enzyme (trypsin) and a protein identification report from a search against the NCBI nonredundant database (which contains all protein sequences from all species). The report will be sent you as a Adobe Acrobat (pdf) file by email. Alternatively we can search only human, bovine, rodent, ecoli, or yeast protein sequences (see check box on sample submission form). If you need your data searched against a different protein sequence database or a specific protein sequence, please forward the sequence or link to the database to tucf-proteomics@tufts.edu.
Volume discount: see volume discount information. Yearly contracts are available (please contact us).
LC/MS/MS only: $150 per sample for protein identification by LC/MS/MS of samples you have already digested.
Liquid (non-gel) samples: For protein identification of liquid samples (in-solution digest), please contact us for pricing and precautions. Contact information at bottom of this page. |
