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Service: Posttranslational Modifications

Please call or email for the additional costs for analysis of post-translation modifications (such as phosphorylation, ubiquitination, etc.). The projects costs and timeframe will vary depending on many factors including sample amount, extent of analysis, etc.. We specialize in finding sites of phosphorylation an ubiquitination (italicize this text) but apply the same techniques to find most any defined posttranslational modification of a protein.

Note:
  • Please call (617) 636-2407 to discuss post-translational modification projects beforehand and pricing. Often the post-translational project will be broken into two steps: protein identification and then determination of modification(s).

  • We will need your protein sequence sent to us by email ahead of time for post-translational projects (we prefer you send the sequence before samples are submitted). Include any tags, cleavages in the sequence, known sequence variations, GST-fusions, etc.

  • Any project involving a non-standard modification (not phosphorylation, oxidation, etc.) should be discussed with us before sending samples. A form may be sent to you where you can provide details of the modification.
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Sample Submission Forms
Use this sample submission form for nanospray LC/MS/MS based analysis for these type of projects:
  • Protein identification from gel bands, spots, or solution
  • Finding sites of posttranslational modification (phosphorylation, ubiquitination, etc.)
  • Protein characterization projects (confirm n- or c-term, confirm sequence variants, etc.)
  • MudPIT multidimensional LC analysis for complex samples (such as lysates)
Protein ID / PTM / MudPIT
PDF
Use this sample submission for all MALDI-TOF projects including:
  • Molecular weight determination for peptides and proteins
  • Examination of degradation products (by molecular weight)
MALDI-TOF
PDF

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Last Modified: Friday, 27-Jun-2008 16:14:08 EDT
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