Please call or email for the additional costs for analysis of post-translation modifications (such as phosphorylation, ubiquitination, etc.). The projects costs and timeframe will vary depending on many factors including sample amount, extent of analysis, etc.. We specialize in finding sites of phosphorylation and ubiquitination, but apply the same techniques to find most any defined posttranslational modification of a protein.
Note:
Please call (617) 636-2407 to discuss post-translational modification projects beforehand and pricing. Often the post-translational project will be broken into two steps: protein identification and then determination of modification(s).
We will need your protein sequence sent to us by email ahead of time for post-translational projects (we prefer you send the sequence before samples are submitted). Include any tags, cleavages in the sequence, known sequence variations, GST-fusions, etc.
Any project involving a non-standard modification (not phosphorylation, oxidation, etc.) should be discussed with us before sending samples. A form may be sent to you where you can provide details of the modification.
We cannot easily look for any or all possible posttranslational modifications of a given protein. You will need to specify which particular posttranslational modifications you want us to try to identify on the protein of interest. If you don’t know which posttranslational modifications your protein has, we suggest you look through literature and come up with a few candidates as it is very time consuming, expensive, and requires a lot of sample to look for more than a few different posttranslational modifications.
Also see the “What’s New” section on the right side of the main webpage to see if there are any delays or if we are currently not accepting new posttranslational modification projects.
